The fixture on May 24 will raise funds for young people in southern Africa.
The Duke of Sussex is to stage his fundraising Sentebale Polo Cup in Italy this year, Buckingham Palace has announced.
The charity match, hosted by the Roma Polo Club on May 24, will help raise funds and awareness for Sentebale, which Harry supports as patron.
Sentebale was founded by the duke and Lesotho’s Prince Seeiso in 2006 to help the most vulnerable children and young people in southern Africa get the support they need to lead healthy and productive lives.
The match will see Harry play on the Sentebale St Regis team captained by Sentebale Ambassador Nacho Figueras, against a US Polo Assn team that will be captained by Malcolm Borwick.